COVID-19 Spike S1 protein:

The binding of spike S protein to the host cell receptor (angiotensin-converting enzyme 2-ACE2) mediates the entry of virus in the host lung tissue. During viral infection, TM protease serine 2 (TMPRSS2), a type 2 TM serine protease located on the host cell membrane, activates the S protein by cleaving it into S1 and S2 subunits, which activate the membrane fusion domain. After that, the viral RNA is copied, producing more viral particles in the host.

The total length of SARS-CoV-2 S protein is 1273 aa. It consists of a signal peptide (amino acids 1–13), the S1 subunit (14–685 residues), and the S2 subunit (686–1273 residues). The spike protein of SARS-CoV-2 is densely glycosylated as it contains 21 to 35 N-glycosylation sites. SARS-CoV-2 uses an extensively glycosylated spike (S) protein that protrudes from the viral surface to bind to angiotensin-converting enzyme 2 (ACE2) to mediate host-cell entry. Understanding the structure and function of SARS-CoV-2 S and the glycosylation of recombinant viral spikes can add value in getting additional information regarding the virus's invasion, pathogenesis, and fundamental features of viral biology, which will support the discovery of antiviral therapeutics and precision vaccine design.

Target: S1 Protein (SARS-CoV2)

Mol. Wt.: 76.7 kDa Glycosylated (Monomer and Trimer Confirmed)

Validation: SDS-PAGE, Immunoblot, MS, ELISA

Lot Sizes

Protein Name : 2019-nCoV Spike S1 Subunit (Glycosylated)
Purity : 90% or above
Size : 0.05 mg, 0.1mg, 0.5mg, 1mg, 10mg
Alternative Name : 2019-nCoV; novel coronavirus spike Protein, 2019-nCoV;
RBD Protein, 2019-nCoV; S1 Protein, 2019-nCoV; Spike RBD Protein, S protein;
S-ECD; ECD protein;
Origin Species :  Coronavirus (2019-nCoV) isolate Wuhan-Hu-1
Expression Host : Pichia pastoris
Protein type : Recombinant
Purification tag : No tag
Molecule Mass : 91 kDa (Glycosylated) Formulation: PBS pH 7.4 containing 10% glycerol
Swiss-prot Accession : SPIKE_SARS2 (confirmed by Mass spectrometry)
Validation : By immunoblot, MS, ELISA (sensitivity ~100ng using RBD specific antibody and convalescent patient sera
Regulation restrictions : For research use only
Shipping : Recombinant proteins are provided as frozen liquid and will be shipped out with dry ice.
Stability and storage : Short term: At 4-8°C Long term: At -20°C Avoid repeated freezing and thawing cycles. The shelf life of the product
is unspecified
Stability and storage : For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Lot Sizes

  • For the development of diagnostic kit (Point of care and ELISA)

  • For serological studies; Protein is being recognized by convalescent patients’ sera

  • For antibody raising

  • Control for bioassays.