Background:
Problem/Rationale:
Solution/Approach:
Figure 1: Steps required to produce clinical grade material
Cell Banking System:
Figure 2: Cell Banking System – Process flow of Cell Banking system as per Q5D
Process Flow:
Figure 3: Process flow diagram for production of clinical grade material
Fermentation Parameters:
Figure 4: Fermentation Parameters – Set point V/S Actual (Temperature, pH & Agitator Speed)
Downstream Processing:
Final Purified Protein: SDS Page
Figure 5: Purified Protein of Interest on 10% SDS Page Gel – M (Marker) & PP (Purified Protein)
The production of recombinant enzymes has led to the development of many diagnostics strategies in various medical fields. The increasing demand for recombinant proteins has created the need to develop efficient protein expression techniques to increase the productivity of the product.
Read MoreVoltage-gated sodium channels are transmembrane proteins responsible for propagating the electrical signal in neurons, cardiac, skeletal muscle, and neuroendocrine cells. Sodium channel Nav1.7 is preferentially expressed in peripheral neurons. Nav1.7 is a validated target for pain treatment in humans. The principal subunit of this channel is a protein of >200 kDa, the alpha subunit, which consists of four large domains of internal homology. The subunit forms the pore and functions in voltage-dependent activation and inactivation,
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